| Vector |
Construct size (kb): 7.300000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-7Zf(+)
Intact vector size: 3.000
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI AsuII ClaII
HindIII BamHI SacI BstXI NsiI
Polylinker sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI AsuII ClaII
HindIII BamHI SacI BstXI NsiI
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--7.0, 1.0; EcoRI--4.7, 3.4; XhoI--4.4, 3.7. Insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.85; BglII--3.76; HindIII--0.20, 0.70, 1.67; KpnI--0.62; NcoI--0.07; PstI--1.40, 3.21; PvuII--0.54, 2.95; XbaI--3.42; XhoI--0.31. Recommendation for verification: EcoRI--4.3, 3.0; BamHI--6.4, 0.9; HindIII--5.6, 1.0, 0.5, 0.2; XbaI--6.4, 0.9; XhoI--4.0, 3.3. |
