Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 0.9, 0.6; PstI--4.4; BamHI/HindIII--2.95, 1.45; XhoI--4.4; XbaI--3.0, 1.4. The insert contains the following restriction sites, in kb from the 5' end: ClaI--0.156; EcoRI--0.906. Isolated from naturally infected Brassica napus ssp. napobrassica (rutabaga) in the region of L'Assomption, Quebec, Canada. Contains the complete capsid protein gene, part of the NIb gene, and part of the 3' end. The 5' end of the insert begins 204 bp 5' to the published sequence and end at nt -506 of the published sequence. Transcription from the T7 promoter gives the plus strand. |
