| 產(chǎn)品名稱 |
Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| 商品貨號 |
B237255 |
| Deposited As |
Saccharomyces cerevisiae Hansen, teleomorph |
| Classification |
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| Strain Designations |
MW3317-21A pms1-delta |
| Alternate State |
Candida robusta Diddens et Lodder |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen |
| Type Strain |
no |
| Genotype |
MATalpha pms1-delta trp1 ade8-deltaKpn ura3-52 hom3-10 met13 met4 ade2 his3-Kpn
Ref Williamson MS, et al. Meiotic gene conversion mutants in Saccharomyces cerevisiae. I. Isolation and characterization of pms1-1 and pms1-2. Genetics 110: 609-646, 1985. PubMed: 3896926, Ref Kramer W, et al. Cloning and nucleotide sequence of DNA mismatch repair gene PMS1 from Saccharomyces cerevisiae: homology of PMS1 to procaryotic MutL and HexB. J. Bacteriol. 171: 5339-5346, 1989. PubMed: 2676974, (Isogenic with MW3317-21A (ATCC 208350) except for the pms locus.) |
| Preceptrol® |
no |
| Mating Type |
alpha |
| Ploidy |
haploid |
| Comments |
Meiosis-sporulation: postmeiotic segregation increase (CL2) |
| Medium |
ATCC® Medium 1069: YPAD medium
|
| Growth Conditions |
Temperature: 25.0°C Atmosphere: Typical aerobic Protocol: The "quick" method for checking pms1 is to print on canavanine media: papillation (mutability) will be increased. The same result is obtained by printing on met- media and treating with UV; increased papillation will occur due to enhanced mutability at the hom3-10 locus in the presence of the pms1 mutation. |
| Subcultivation |
Protocol: The "quick" method for checking pms1 is to print on canavanine media: papillation (mutability) will be increased. The same result is obtained by printing on met- media and treating with UV; increased papillation will occur due to enhanced mutability at the hom3-10 locus in the presence of the pms1 mutation. |
| Name of Depositor |
YGSC |
| Special Collection |
Yeast Genetic Stock Center |
| Chain of Custody |
ATCC <<--YGSC<<--W. Kramer and K. Lusnak |
| References |
Williamson MS, et al. Meiotic gene conversion mutants in Saccharomyces cerevisiae. I. Isolation and characterization of pms1-1 and pms1-2. Genetics 110: 609-646, 1985. PubMed: 3896926
Kramer W, et al. Cloning and nucleotide sequence of DNA mismatch repair gene PMS1 from Saccharomyces cerevisiae: homology of PMS1 to procaryotic MutL and HexB. J. Bacteriol. 171: 5339-5346, 1989. PubMed: 2676974
Isogenic with MW3317-21A (ATCC 208350) except for the pms locus.
Williamson MS, et al. Meiotic gene conversion mutants in Saccharomyces cerevisiae. I. Isolation and characterization of pms1-1 and pms1-2. Genetics 110: 609-646, 1985. PubMed: 3896926
Kramer W, et al. Cloning and nucleotide sequence of DNA mismatch repair gene PMS1 from Saccharomyces cerevisiae: homology of PMS1 to procaryotic MutL and HexB. J. Bacteriol. 171: 5339-5346, 1989. PubMed: 2676974
Isogenic with MW3317-21A (ATCC 208350) except for the pms locus.
|