| 產品名稱 |
Raccoonpox virus |
| 商品貨號 |
B237272 |
| Classification |
Poxviridae, Orthopoxvirus |
| Deposited As |
Raccoonpox virus, Orthopoxvirus |
| Agent |
Raccoonpox virus |
| Strain |
RCN KB3-JE-13 (rabG) [CVS Rabiesvirus Glycoprotein recombinant] |
| Applications |
Raccoon poxvirus CDC # V71-I-85A was plaque purified three times then plasmid pKB3-JE-13 (ATCC 40280) was transfected to cause insertion of CVS rabies glycoprotein cDNA in the thymidine kinase gene, inactivating it. |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
Recombinant Rabies vaccine |
| Product Format |
frozen |
| Storage Conditions |
-70° or colder |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Comments |
Raccoon poxvirus CDC # V71-I-85A was plaque purified three times then plasmid pKB3-JE-13 (ATCC 40280) was transfected to cause insertion of CVS rabies glycoprotein cDNA in the thymidine kinase gene, inactivating it. Plaque titration is on CV-1, BSC40 or Rat2 cells. Adsorb virus for 2 hours at 36C, overlay with DMEM + 2% FBS (liquid overlay is OK) incubate for 72 hours, stain monolayer with 0.01% crystal violet in 10% formalin, rinse with PBS. |
| Effect on Host |
Yes, in vitro effects: Cytopathic effects (cell rounding, syncytium formation) |
| Recommended Host |
CV-1 cells (ATCC CCL-70); Rat2 cells (ATCC CRL-1764; BSC40 cells primate cell lines; Vero cells (ATCC CCL-81); HeLa cells (ATCC CCL-2); chicken chorioallantoic membrane; rat 2 (TK-) cells |
| Growth Conditions |
Temperature: 36°C Incubation: incubate at 36°C |
| Name of Depositor |
JJ Esposito |
| U.S. Patent |
|
| U.S. Patent Number |
|
| Source |
Recombinant Rabies vaccine |
| References |
Esposito JJ, Baer GM. Raccoon poxvirus as a gene expression and vaccine vector for genes of rabies virus and other organisms. US Patent 5,266,313 dated Nov 30 1993
Esposito JJ, et al. Successful oral rabies vaccination of raccoons with raccoon poxvirus recombinants expressing rabies virus glycoprotein. Virology 165: 313-316, 1988. PubMed: 3291388
EspositoModern approaches to new vaccines including prevention of AIDS.In: EspositoVaccines 92321-330, 1992
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