| Insert |
DNA: genomic Insert lengths(kb): 9.5 Tissue: fetal liver Gene product: alpha-fetoprotein( alpha-1-fetoprotein, alpha-foetoprotein, alpha-fetoglobulin) [AFP] Alleles: B1, B2, C1, C2, D1, D2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--14.3; EcoRI--10.0, 4.3; PstI--5.3, 4.3 (doublet), 0.45; SalI--14.3; HindIII--5.3 (doublet), 1.5, 1.0, 0.56. The insert contains the following restriction sites (nt position according to the published sequence): PstI--10769, 16150; HindIII--15707, 16270, 17864, 18893. This is the EcoRI fragment extending from nt 10198 to 19732 of the published sequence. Subcloned from lambdaHAFP26 (ATCC 65736, 65737). Contains exons 8-13 and two "Xba" repeats. |
| References |
Gibbs PE, et al. Structure, polymorphism, and novel repeated DNA elements revealed by a complete sequence of the human alpha-fetoprotein gene. Biochemistry 26: 1332-1343, 1987. PubMed: 2436661
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