Features: marker(s): ampR promoter: SP6 replicon: pMB1, f1
Applications
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
Comments
M9 medium is required to retain f1.
This plasmid vector can be used for in vitro RNA synthesis from the SP6 promoter.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
M9
References
Mead DA, et al. Single stranded DNA SP6 promoter plasmids for engineering mutant RNAs and proteins: synthesis of a 'stretched' preproparathyroid hormone. Nucleic Acids Res. 13: 1103-1118, 1985. PubMed: 3858795
