| 產(chǎn)品名稱(chēng) |
BDCM |
| 商品貨號(hào) |
B237868 |
| Organism |
Homo sapiens, human |
| Tissue |
peripheral blood |
| Cell Type |
B lymphoblast |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
2 [Cells contain EBV viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
acute myelogenous leukemia (AML) |
| Age |
unknown |
| Gender |
male |
| Ethnicity |
unknown |
| Applications |
Cells may be useful in modeling immune reactions. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
BDCM is a B cell line established from a pheresis sample from a patient with M5a leukemia. |
| Clinical Data |
male
unknown |
| Antigen Expression |
CD20; Homo sapiens, expressed
CD19; Homo sapiens, expressed
CD40; Homo sapiens, expressed
CD80; Homo sapiens, expressed
CD83; Homo sapiens, expressed
CD86; Homo sapiens, expressed
HLA-DR; Homo sapiens, expressed
CD1a; Homo sapiens, not expressed
CD11c; Homo sapiens, not expressed |
| Receptor Expression |
interleukin 3 (IL-3) |
| Comments |
In addition to B cell markers, the cells have many characteristics of dendritic cells.
The cells are positive for Epstein-barr virus (EBV).
They produce fascin, interleukin 1 alpha (IL-1 alpha), interleukin 2 (IL-2), interleukin 6 (IL-6), interleukin 16 (IL-16), transforming growth factor beta1 (TGF beta1), and tumor necrosis factor (TNF).
|
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 105 cells/mL and maintain between 3 X 105 and 3 X 106 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 10,11 D13S317: 11 D16S539: 10,11 D5S818: 12,13 D7S820: 9,11 THO1: 6,9.3 TPOX: 9,10 vWA: 16,17 |
| Name of Depositor |
RA Steinman |
| Deposited As |
human |
| References |
Kharbanda S, et al. BDCM: a novel B-cell line with genetic and functional similarity to dendritic cells. Br. J. Haematol. 119: 819-825, 2002. PubMed: 12437665
|
