| 產(chǎn)品名稱 |
Cochliobolus sativus (Ito et Kuribayashi) Drechsler ex Dastur |
| 商品貨號(hào) |
B239058 |
| Strain Designations |
ND90Pr |
| Alternate State |
Bipolaris sorokiniana (Saccardo) Shoemaker, anamorph |
| Application |
Tester for mating type A |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen |
| Storage Conditions |
Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section |
| Type Strain |
no |
| Genotype |
pathotype 2 |
| Preceptrol® |
no |
| Genome Sequenced Strain |
Yes
|
| Mating Type |
MAT-2 (or A) |
| Comments |
differential virulence Genome sequencing strain (the Joint Genome Institute at the Department of Energy, USA). |
| Morphology |
After 5 days at 20°C colonies floccose with grey aerial mycelium and reverse dark brown to black.? Later conidia curved to straight, fusiform to broadly ellipsoidal, 3-12 distoseptate, 40-120 x 17-28 μm. |
| Medium |
ATCC® Medium 350: Emerson YpSs agar
ATCC® Medium 336: Potato dextrose agar (PDA)
ATCC® Medium 28: Emmons' modification of Sabouraud's agar
|
| Growth Conditions |
Temperature: 24°C to 26°C Atmosphere: Typical aerobic |
| Sequenced Data |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence.
GCCTGGCTTCGCGGCCGGCTGAAATATTTTTTTCACCCATGTCTTTTGCGCACTTGTTGTTTCCTGGGCGGGTTCGCCCGCCACCAGGACCAAACCATAAACCTTTTTTTTATGCAGTTGCAATCAGCGTCAGTAAAAACAATGTAATTATTACAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATACGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCAAAGGGCATGCCTGTTCGAGCGTCATTTGTACCTTCAAGCTTTGCTTGGTGTTGGGCGTTTTTTGTCTCCCTCTTTCTGGGAGACTCGCCTTAAAACGATTGGCAGCCGGCCTACTGGTTTCGGAGCGCAGCACATATTTTGCGCTTTGTATCAGGAGAAAAGGACGGTAATCCATCAAGACTCTACATTTTTAACTTTTGACCTCGGATCAGGTAG
D1D2 region of the 28S ribosomal RNA gene
ATTGCCCTAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCTCTTTCAGAGTCCGAGTTGTAATTTGCAGAGGGCGCTTTGGCTTTGGCAGCGGTCCAAGTTCCTTGGAACAGGACGTCACAGAGGGTGAGAATCCCGTACGTGGTCGCTAGCTATTGCCGTGTAAAGCCCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGAGGTAAATTTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTCAAACAGCACGTGAAATTGTTGAAAGGGAAGCGCTTGCAGCCAGACTTGCTTGCAGTTGCTCATCCGGGCTTTTGCCCGGTGCACTCTTCTGTAGGCAGGCCAGCATCAGTTTGGGCGGTGGGATAAAGGTCTCTGTCACGTACCTCTCTTCGGGGAGGCCTTATAGGGGAGACGACATACCACCAGCCTAGACTGAGGTCCGCGCATCTGCTAGGATGCTGGCG
|
| Name of Depositor |
BJ Steffenson |
| Isolation |
Barley leaves, Hordeum vulgare line I86-519-1, Prosper, Cass Co., ND |
| Cross References |
Nucleotide (GenBank) :
JQ070161
D1/D2 region of 28S rRNA gene
Nucleotide (GenBank) :
JQ070090
ITS including 5.8S rRNA gene
Nucleotide (GenBank) :
AEIN00000000
Bipolaris sorokiniana ND90Pr, whole genome shotgun sequencing project
|
| References |
Fetch TG Jr., Steffenson BJ. Identification of Cochliobolus sativus isolates expressing differential virulence on two-row barley genotypes from North Dakota. Can. J. Plant Pathol. 16: 202-206, 1994.
Valjavec-Gratian M, Steffenson BJ. Genetics of virulence in Cochliobolus sativus and resistance in barley. Phytopathology 87: 1140-1143, 1997.
Valjavec-Gratian M, Steffenson BJ. Pathotypes of Cochliobolus sativus on barley in North Dakota. Plant Dis. 81: 1275-1278, 1997.
Zhong S, Steffenson BJ. Genetic and molecular characterization of mating type genes in Cochliobolus sativus. Mycologia 93: 852-863, 2001.
Ohm RA, et al. Diverse lifestyles and strategies of plant pathogenesis encoded in the genomes of eighteen Dothideomycetes fungi. PLoS Pathog 8: e1003037, 2012. PubMed: 23236275
|
