Restriction digests of the clone give the following sizes (kb): HindIII--2.55, 2.4, 2.2 (doublet), 2.15, 1.1; BamHI--uncut; ClaI--12.1, ClaI/BamHI--12.1. Can be mobilized into F. diplosiphon using the conjugative plasmid RP4. Shuttle vector. A 2.9 kb BamHI/ClaI fragment from a different construct containing the origin of replication has been sequenced. The BamHI site is not retained in this plasmid. Constructed by inserting a 8.0 kb ClaI fragment, containing the F. diplosiphon pFDA origin of replication, into the AsuII site of pJCF22. The order of the major features of the plasmid is: kanR - cmlR - F. diplosiphon pFDA ori - bom - oriV. |
