Restriction digests of the clone give the following sizes (kb): BamHI/PvuII--4.8, 2.6, 0.5, 0.46; EcoRI--8.4; SmaI--8.4. Shuttle expression vector which includes a reporter group. Expresses beta-galactosidase activity in E. coli, S. cerevisiae, and mouse cells, and thus can be used to calibrate transformation efficiency. Also permits blue/white detection in E. coli. The EcoRI, SmaI, and BamHI sites are 3' to the unique available in-phase initiation codon under control of the SV40 early promoter. Constructed by inserting a 3.048 kb EcoRI/DraI fragment from pMC1403 into pCYM1 cut with BamHI (nt 427), linkered to add an EcoRI site, and cut with BalI (nt 702). |
