| 產品名稱 |
Simian virus 40 |
| 商品貨號 |
B241109 |
| Designations |
pUCSV-VA45-54-2Ea [molecular clone of Simian virus 40, SV-40] |
| GenBank Number |
AF156105
|
| Species |
Simian virus 40, Simian virus 40 |
| Depositors |
JA Lednicky, JS Butel |
| Applications |
contains sequence |
| Vector |
Construct size (kb): 7.949999809265137 |
| Insert |
DNA: cDNA Insert lengths(kb): 5.263999938964844 Tissue: uninoculated grivet monkey cells Gene product: [SV-VA45-54-2Ea] |
| Insert Size (kb) |
5.264 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Insert contains following restriction sites (from 5'end): BamHI-0.771,7.179; EcoRI-2.686,5.264; KpnI-3.786,4.164; SalI-7.950; SfiI-7.950; XhoI-uncut. Plasmid size: 7950bp (2686bp pUC-19vector+ 5264bp SV40-VA45-54-2 insert). This variant of SV-40-VA45-54 has an non-archetypal viral regulatory region and was verified by lipofection of EcoRI-linearized plasmid pUCSV-VA45-54-2Ea DNA into ATCC CV-1 cells by the contributor, followed by DNA sequence analysis of the virus stock. |
| Classification |
Polyomaviridae, Polyomavirus |
| References |
Lednicky JA, Butel JS. Tissue culture adaptation of natural isolates of simian virus 40: changes occur in viral regulatory region but not in carboxy-terminal domain of large T-antigen. J. Gen. Virol. 78: 1697-1705, 1997. PubMed: 9225047
Stewart AR, et al. Sequence analyses of human tumor-associated SV40 DNAs and SV40 viral isolates from monkeys and humans. J. Neurovirol. 4: 182-193, 1998. PubMed: 9584955
|
