XbaI, SmaI, SstI and EcoRI appear in both the polylinker and the aph cassette. Restriction digests of the clone give the following sizes (kb): HindIII--4.8; EcoRI--4.7, 0.2. An Escherichia coli/eukaryotic cell line shuttle vector containing lacZ and multiple cloning sites of pUC18 and the aph (neoR) gene under the control of murine sarcoma virus promoter and enhancer. The PvuII at position 628 of pUC18 was changed to BglII. A cassette containing the aph gene under control of murine sarcoma virus promoter and enhancer was inserted at the BglII site. Aph is transcribed in the opposite direction of lacZ. |
