| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--24.0, 19.5, 0.8; BamHI--21.0, 11.5, 6.4, 5.4; HindIII--27.0, 9.6, 4.4, 3.8; PstI--10.5, 8.6, 4.8, 2.8, 2.5, 2.1, 1.9, 1.7, 1.3, 1.1, 0.7 + smaller; SmaI--37.5, 5.5. With the G of the EcoRI site being nucleotide 1, the initiation ATG of this insert is nucleotide 12. This is a 5' probe containing only 5 bp of 5' untranslated sequence. |
| References |
Srivastava PK, et al. 5'-structural analysis of genes encoding polymorphic antigens of chemically induced tumors. Proc. Natl. Acad. Sci. USA 84: 3807-3811, 1987. PubMed: 2438686
Pramod K Srivastava, personal communication
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