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in suitable host, produces protein A-kinase anchor protein 100

Construct size (kb): 7.639999866485596

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pET11d-kfc
Intact vector size: 5.800
Type of vector: plasmid
Vector end: NcoI
Vector end: BamHI
Cloning sites: NcoI BamHI
Polylinker sites:
Construction: pET11d
Host range: Escherichia coli
Features (with orientation and position when available):
transcription terminator: T7, <-
other: calmodulin binding domain, <-
restriction site: KpnI
restriction site: BglII
other: factor X unit, <-
restriction site: SalI
other: kemptide unit, <-
restriction site: BamHI
epitope tag: T7-tag coding sequence, <-
restriction site: NcoI
promoter for in vitro transcription: T7, <-
repressor gene: lacI, ->
replicon: pMB1
marker(s): ampR, <-
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: AKAP100*
Gene name: A-kinase anchor protein 100
Contains complete coding sequence?: Y
Tissue: hippocampus
Type of DNA: cDNA
Insert 5' end: NcoI
Insert 3' end: BamHI
Insert size (kb): 1.965
Cross references: DNA Seq. Acc.: U17195
Nucleotides 1-1965 of the insert correspond to
nucleotides 1-1965 of U17195.

Insert lengths(kb): 1.9650000333786

Tissue: hippocampus

Gene product: A-kinase anchor protein 100 [AKAP100*]

Target Gene: A-kinase anchor protein 100

Restriction digests of the clone give the following sizes (kb): BamHI/NcoI--5.8, 2.0; EcoRI--6.0, 1.8; HindIII--7.0, 0.6; PstI--6.2, 1.6; PvuII--3.0, 2.8, 1.5, 0.3.

Constructed by ligating a PCR product creating an NcoI site on the 5' end to a fragment from a cDNA library.

The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--0.60; HindIII--1.75; NdeI--0.67; PstI--1.55; PvuII--1.31, 1.62; SacI--0.09.

Transcription from the T7 promoter allows expression of a kfc-fusion protein that can be purified by affinity chromatography on calmodulin-agarose. (kfc refers to kemptide - factor X - calmodulin-binding domains).