Insert contains the following sites separated by (kb): PstI - 0.45 - PstI - 0.2 - EcoRI - 0.71 - KpnI - 0.59 - HincII - 0.43 - HincII - 0.52 - HindIII - 0.32 - PstI. The 0.52 and 0.32 fragments have internal AvaI sites. Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 3.35; HindIII--6.6, 1.1; PstI--4.5, 2.8, 0.4. This contains two inserts of 2770 bp and 450 bp of Arabis mosaic virus RNA2. The entire virus is 4060 nt in length. Isolated by W. Jelkmann. |
