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Vaccinia virus ts mutant

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編號:B243164

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產(chǎn)品名稱	Vaccinia virus ts mutant
商品貨號	B243164
Classification	Poxviridae, Orthopoxvirus
Agent	Vaccinia virus ts mutant
Strain	IHD-W Dts47 (Dales isolate 6828)
Applications	The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U12.
Positive for Mycoplasma	No
Biosafety Level	2 CDC BMBL recommends vaccination. ref Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation	derived from existing strain
Product Format	frozen
Storage Conditions	-70°C or colder (or colder)
Comments	Selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. The non-permissive incubation temperature is 39.5oC. This mutant was assigned a Dales EM category of P, characterized by immature normal particles and mature particles with cores that are predominantly lucent at the center. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U12.
Effect on Host	Yes, Cytopathic effect: Rounding and sloughing of infected cells CPE: enlargement, plaques with rounding and eventual sloughing
Growth Medium	Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
Recommended Host	Recommended Host: BSC-40 (ATCC^? CRL-2761
Growth Conditions	Atmosphere: 5% CO2 in air recommended Temperature: 31.0°C Duration: 1-3 days; For best results, cells should be 24 to 48 hours old and 80-90% confluent [not 100% confluent] for inoculation.
Cryopreservation	Storage temperature: -70°C
Name of Depositor	S Dales, RC Condit, S Dales
Special Collection	ATCC
Source	derived from existing strain
References	Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807 Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746 This mutant strain was derived by Samuel Dales from the IHD-W strain of Vaccinia (ATCC VR-1441) which in turn was derived from the IHD-J strain. Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570 Vaccination recommended for laboratory staff manipulating vaccinia, monkeypox, or cowpox viruses [CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4]. or colder Non-permissive incubation temperature is 39.5C. This mutant was assigned a Dales EM category of P. It is assigned the Condit map location U12. This subtype is distinguishable from the parent IHD-J strain by its ability to elicit polykaryocytosis in all mammalian and avian cells tested and by its inability to induce an active hemagglutinin at the plasma membrane. or colder

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