Restriction digests of the clone give the following sizes (kb): HindIII--3.5; XmnI--2.4, 1.1; SmaI--3.3, 0.15; EcoRI--2.9, 0.6; PstI--3.5. The insert contains the following restriction sites (nt from the 5' end): SphI--17, 86; SmaI--134. Contains the complete coding sequence, and corresponds to nt 1-545 of the database record. The sense strand is packaged. pEM301 (ATCC 79032/3) and pEM302 (79034/5) differ only in the orientation of the insert. |
