Restriction digests of the clone give the following sizes (kb): EcoRI--5.5; BamHI--5.5; PstI--5.5; HindIII--5.5. Shuttle vector permitting visual detection of recombinants by beta-galactosidase alpha complementation. Constructed by inserting a 1.7 kb ClaI fragment containing ura4 and a 1.2 kb ClaI fragment containing ars1 into the NdeI site modified with a ClaI linker. The order of the major features in this plasmid is: ars1 - ura4 - HindIII/MCS/EcoRI/lacZ' - pMB1 ori - ampR. |
